CLONAL GROWTH IN VITRO OF HUMAN CELLS WITH FIBROBLASTIC MORPHOLOGY
نویسندگان
چکیده
منابع مشابه
Clonal Growth in Vitro of Human Cells with Fibroblastic Morphology
A methodology has been described for reliable cultivation in vitro of dispersed fibroblastic cells obtained from normal human organs. The procedure has permitted establishment of stable cell lines from almost every sample taken, among which the following organs were represented: skin, spleen, amnion, lung, liver, bone marrow, brain, muscle, and heart. Equally good growth has been achieved with ...
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Two methods for simple and rapid plating of single HeLa cells, human, carcinomatous cells, are described. These result in growth and formation of colonies from each single cell. One of these procedures uses irradiated, non-multiplying "feeder" cells to condition the medium. The second requires more gentle handling of the cells, but otherwise is virtually the same as that used in plating bacteri...
متن کاملCLONAL GROWTH IN VITRO OF HUMAN CELLS W I T H FIBROBLASTIC MORPHOLOGY*, :~ COMPARISON OF GROWTH AND GENETIC CHARACTERISTICS Ol~ SINGLE EPITktELIOID AND FIBROBLAST-LIKE CELLS FROM A VARIETY O~' HUMAN ORGANS
In previous papers (1-3) we have described methods for plating single human cells so that each multiplies in isolation to form a well defined colony which constitutes a clonal population. The procedure is simple and quantitative, and completely comparable to the standard plating procedure used by microbiologists for growth of colonies from single bacterial cells. In the work published thus far ...
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Tissue culture strains of cells from four different normal human tissues-liver, conjunctiva, kidney, and appendix-have been grown by the plating procedure previously developed for the HeLa strain of cervical carcinoma cells. This technique results in colony formation from isolated single cells, in a manner completely analogous to the plating of bacteria in semisolid nutrient media. Clonal cell ...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1957
ISSN: 1540-9538,0022-1007
DOI: 10.1084/jem.106.1.145